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Allergen-specific oral immunotherapy for peanut allergy
(Review)
Nurmatov U, Venderbosch I, Devereux G, Simons FER, Sheikh A
This is a reprint of a Cochrane review, prepared and maintained by The Cochrane Collaboration and published in The Cochrane Library2012, Issue 9
http://www.thecochranelibrary.com
Allergen-specific oral immunotherapy for peanut allergy (Review)
Copyright © 2012 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd.
T A B L E O F C O N T E N T S
1HEADER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1ABSTRACT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2PLAIN LANGUAGE SUMMARY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3BACKGROUND . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
4OBJECTIVES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
4METHODS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6RESULTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
8DISCUSSION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
9AUTHORS’ CONCLUSIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
9ACKNOWLEDGEMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
9REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
12CHARACTERISTICS OF STUDIES . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
20DATA AND ANALYSES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
20ADDITIONAL TABLES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
20APPENDICES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
23HISTORY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
23CONTRIBUTIONS OF AUTHORS . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
23DECLARATIONS OF INTEREST . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
24SOURCES OF SUPPORT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
24DIFFERENCES BETWEEN PROTOCOL AND REVIEW . . . . . . . . . . . . . . . . . . . . .
iAllergen-specific oral immunotherapy for peanut allergy (Review)
Copyright © 2012 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd.
[Intervention Review]
Allergen-specific oral immunotherapy for peanut allergy
Ulugbek Nurmatov1, Iris Venderbosch2, Graham Devereux3, F Estelle R Simons4, Aziz Sheikh5
1Allergy & Respiratory Research Group, Centre for Population Health Sciences, The University of Edinburgh, Edinburgh, UK.2Radboud University Nijmegen Medical Center, Nijmegen, Netherlands. 3Department of Child Health, Royal Aberdeen Children’s
Hospital, The University of Aberdeen, Aberdeen, UK. 4Department of Pediatrics & Child Health; Department of Immunology, Faculty
of Medicine, University of Manitoba, Winnipeg, Canada. 5Centre for Population Health Sciences, The University of Edinburgh,
Edinburgh, UK
Contact address: Aziz Sheikh, Centre for Population Health Sciences, The University of Edinburgh, Medical School, Doorway 3,
Teviot Place, Edinburgh, EH8 9AG, UK. [email protected].
Editorial group: Cochrane Tobacco Addiction Group.
Publication status and date: New, published in Issue 9, 2012.
Review content assessed as up-to-date: 13 April 2012.
Citation: Nurmatov U, Venderbosch I, Devereux G, Simons FER, Sheikh A. Allergen-specific oral immunotherapy for peanut allergy.
Cochrane Database of Systematic Reviews 2012, Issue 9. Art. No.: CD009014. DOI: 10.1002/14651858.CD009014.pub2.
Copyright © 2012 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd.
A B S T R A C T
Background
Peanut allergy is one of the most common forms of food allergy encountered in clinical practice. In most cases, it does not spontaneously
resolve; furthermore, it is frequently implicated in acute life-threatening reactions. The current management of peanut allergy centres
on meticulous avoidance of peanuts and peanut-containing foods. Allergen-specific oral immunotherapy (OIT) for peanut allergy
aims to induce desensitisation and then tolerance to peanut, and has the potential to revolutionise the management of peanut allergy.
However, at present there is still considerable uncertainty about the effectiveness and safety of this approach.
Objectives
To establish the effectiveness and safety of OIT in people with IgE-mediated peanut allergy who develop symptoms after peanut
ingestion.
Search methods
We searched in the following databases: AMED, BIOSIS, CAB, CINAHL, The Cochrane Library, EMBASE, Global Health, Google
Scholar, IndMed, ISI Web of Science, LILACS, MEDLINE, PakMediNet and TRIP. We also searched registers of on-going and
unpublished trials. The date of the most recent search was January 2012.
Selection criteria
Randomised controlled trials (RCTs), quasi-RCTs or controlled clinical trials involving children or adults with clinical features indicative
of IgE-mediated peanut allergy treated with allergen-specific OIT, compared with control group receiving either placebo or no treatment,
were eligible for inclusion.
Data collection and analysis
Two review authors independently checked and reviewed titles and abstracts of identified studies and assessed risk of bias. The full text
of potentially relevant trials was assessed. Data extraction was independently performed by two reviewers with disagreements resolved
through discussion.
1Allergen-specific oral immunotherapy for peanut allergy (Review)
Copyright © 2012 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd.
Main results
We found one small RCT, judged to be at low risk of bias, that enrolled 28 children aged 1 to 16 years with evidence of sensitisation
to peanut and a clinical history of reaction to peanut within 60 minutes of exposure. The study did not include children who had
moderate to severe asthma or who had a history of severe peanut anaphylaxis. Randomisation was in a 2:1 ratio resulting in 19 children
being randomised to the intervention arm and nine to the placebo arm. Intervention arm children received OIT with peanut flour and
control arm participants received placebo comprising of oat flour. The primary outcome was assessed using a double-blind, placebo
controlled oral food challenge (OFC) at approximately one year. No data were available on longer term outcomes beyond the OFC
conducted at the end of the study.
Because of adverse events, three patients withdrew from the intervention arm before the completion of the study. Therefore, only 16
participants received the full course of peanut OIT, whereas all nine patients receiving placebo completed the trial. The per-protocol
analysis found a significant increase in the threshold dose of peanut allergen required to trigger a reaction in those in the intervention
arm with all 16 participants able to ingest the maximum cumulative dose of 5000 mg of peanut protein (which the authors equate as
being equivalent to approximately 20 peanuts) without developing symptoms, whereas in the placebo group they were able to ingest
a median cumulative dose of 280 mg (range: 0 to 1900 mg, P < 0.001) before experiencing symptoms. Per-protocol analyses also
demonstrated that peanut OIT resulted in reductions in skin prick test size (P < 0.001), interleukin-5 (P = 0.01), interleukin-13 (P =
0.02) and an increase in peanut-specific immunoglobulin G4 (IgG4) (P < 0.01).
Children in the intervention arm experienced more adverse events during treatment than those in the placebo arm. In the initial day
escalation phase, nine (47%) of the 19 participants initially enrolled in the OIT arm experienced clinically-relevant adverse events
which required treatment with H1-antihistamines, two of which required additional treatment with epinephrine (adrenaline).
Authors’ conclusions
The one small RCT we found showed that allergen-specific peanut OIT can result in desensitisation in children, and that this is
associated with evidence of underlying immune-modulation. However, this treatment approach was associated with a substantial risk
of adverse events, although the majority of these were mild. In view of the risk of adverse events and the lack of evidence of long-
term benefits, allergen-specific peanut OIT cannot currently be recommended as a treatment for the management of patients with IgE-
mediated peanut allergy. Larger RCTs are needed to investigate the acceptability, long-term effectiveness and cost-effectiveness of safer
treatment regimens, particularly in relation to the induction of clinical and immunological tolerance.
P L A I N L A N G U A G E S U M M A R Y
Oral immunotherapy for the treatment of peanut allergy
Allergy to peanut can result in potentially life-threatening reactions and, on occasions, death. Unlike many other forms of food allergy,
allergy to peanut is typically life-long. There is currently no cure for peanut allergy and people with this allergy must constantly be
careful to avoid accidentally eating peanut or peanut-containing foods. If a person with a peanut allergy accidentally ingests peanut,
he or she may develop serious allergic reactions necessitating emergency treatment with epinephrine (adrenaline).
The overall goal of allergen-specific oral immunotherapy (OIT) for peanut allergy is to reduce and, if possible, eliminate the risk
of further reactions associated with exposure to peanuts. Most people who have reactions to peanut have an immediate type (also
sometimes known as IgE-mediated) reaction in which symptoms typically develop within minutes of exposure to peanut protein and
it is for this group that allergen-specific OIT is a potential treatment approach. It is not considered suitable for those who experience
more delayed (also sometimes known as non-IgE mediated) reactions. Treatment involves giving people with peanut allergy very small
doses of peanut protein by mouth and gradually increasing the amount being administered (the build-up phase). Once the desired
dose has been achieved, this is followed by the patient taking the same dose of peanut every day for a set period of time (maintenance
phase). As giving doses of peanut to someone with a peanut allergy has the potential to introduce allergic reactions, participants’ safety
needs to be carefully monitored during studies of peanut OIT.
We found one small trial undertaken in 28 children aged 1 to 16 years with confirmed peanut allergy. The study did not include children
who had moderate to severe asthma or who had had severe anaphylaxis (a severe allergic reaction that may result in death) because
of their peanut allergy. The authors randomised children to intervention or placebo in a 2:1 ratio. Intervention arm children received
peanut flour whereas control arm participants received oat flour. The 48-week trial showed that treatment with peanut OIT enabled
children receiving OIT to substantially increase the amount of peanut flour they ate in comparison with those in the placebo arm
2Allergen-specific oral immunotherapy for peanut allergy (Review)
Copyright © 2012 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd.
without having an allergic reaction. However, almost half of the children (nine out of 19) receiving OIT had an allergic reaction due to
the OIT which required antihistamines, and two had more serious reactions to the treatment which required adrenaline (epinephrine).
Although promising, based on the findings of this one small trial, we cannot recommend that peanut OIT be used routinely for
people with peanut allergy. There is a need for further larger studies investigating safer OIT regimens and establishing the long-term
effectiveness of OIT after treatment is stopped.
B A C K G R O U N D
Description of the condition
Peanut allergy is one of the most common food allergies in west-
ernised countries affecting, for example, up to 1.8% of young
children in the United Kingdom (Hourihane 2007) and approx-
imately 1% of children and 0.6% of adults in North America
(Sicherer 2010b). The prevalence of challenge-proven peanut al-
lergy was 3.0% in Australian infants (Osborne 2011). The preva-
lence of peanut allergy may (Mullins 2009; Sicherer 2010a) or
may not (Ben-Shoshan 2009; Kotz 2011) be increasing.
Of the eight major allergenic proteins in peanut, the components
Ara h1 and Ara h2 (particularly the latter) are the most impor-
tant predictors of clinical symptoms on exposure (de Leon 2007;
Nicolaou 2010).
IgE antibody-mediated allergic reactions to peanut involve specific
sensitisation of tissue mast cells and blood basophils by the binding
of IgE to the high-affinity IgE receptors on these cells. On re-
exposure, peanut proteins bind to the IgE antibody specific to them
and this triggers the release of histamine, tryptase and a variety
of other inflammatory mediators (Burks 2008). Symptoms of an
acute allergic reaction typically develop within minutes to a few
hours after peanut ingestion. Severity is unpredictable and ranges
from mild skin symptoms (e.g. hives, flushing), gastrointestinal
symptoms (e.g. vomiting, abdominal pain, diarrhoea), to severe
or fatal anaphylaxis involving obstruction of the upper or lower
airways and respiratory distress and/or cardiovascular collapse (
Burks 2008; Simons 2011; Soar 2008 ).
There is currently no disease-modifying treatment or cure for
peanut allergy (Sheikh 2010). Current management involves strict
avoidance of peanut in all forms, including the minute quan-
tities present in many packaged foods, as well as more obvious
sources such as desserts, cookies and candies. Accidental expo-
sures occur annually in more than 15% of patients at risk (Clark
2008; Yu 2006). The quality of life of affected people and their
caregivers can be substantially reduced due to fear of incorrect
food choices, ingestion of food containing hidden allergens, and
the ever-present threat of anaphylaxis (Akeson 2007; King 2009;
Cummings 2010).
Pharmacological treatment of peanut allergy involves teaching
patients and caregivers to recognise symptoms of anaphylaxis
(Gallagher 2011), to promptly use an epinephrine (adrenaline)
auto-injector, and to activate emergency medical services (Simons
2009).
Clinical peanut allergy is typically life-long (Sicherer 2007). Ther-
apeutic interventions that provide permanent protection against
unintentional peanut ingestion are therefore needed. Two decades
ago, placebo-controlled trials of subcutaneous immunotherapy
in patients with clinical peanut allergy showed promising re-
sults, but there was an unacceptably high rate of adverse events
(Oppenheimer 1992; Nelson 1997). Peanut oral immunotherapy
(OIT) has been studied recently in patients with clinical peanut
allergy, building on an approach successfully used more than a cen-
tury ago to desensitise a child with egg allergy (Schofield 1908).
In peanut OIT, incremental doses of peanut protein are admin-
istered to carefully selected patients in physician-monitored, con-
trolled clinical settings. Up-dosing in such settings is interspersed
with periods of maintenance dosing at home (Burks 2008; Sicherer
2010b). The initial aim however is to provide clinical desensitisa-
tion - i.e. to increase the threshold dose of exposure to peanut, and
reduce the risk of allergic reactions from unintentional ingestion
of peanut. The ultimate aim is to produce clinical and immuno-
logic tolerance through down-regulation of the Th2 response to
peanut that will endure irrespective of whether a previously clini-
cally reactive patient continues to eat peanut on a regular basis or
does not eat peanut at all (Clark 2009; Jones 2009).
There are different types/regimens of OIT protocols (e.g. rush,
slow). In peanut OIT, peanut allergen can be delivered in a range
of forms (e.g. crushed roasted peanuts, peanut flour and whole
peanut kernels) and is swallowed thereby facilitating contact of
the allergen with the gastro-intestinal mucosa.
Three non-randomised open studies of peanut OIT have been
published to-date (Blumchen 2010; Clark 2009; Jones 2009) and
the findings from these have been synthesised in an earlier system-
atic review (Sheikh 2012). Although at inherently high risk of bias,
these studies suggested that peanut OIT may achieve desensitisa-
tion. For example, in one study, peanut OIT resulted in clinical
desensitisation (defined as the ability to tolerate 3.9 g of peanut
protein during an oral food challenge) for 27 of the 29 children
3Allergen-specific oral immunotherapy for peanut allergy (Review)
Copyright © 2012 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd.
who completed at least eight months of treatment. Down-regula-
tion of the Th2 response to peanut was also documented: by 6 to
12 months, the size of the peanut skin prick test (SPT) decreased,
basophil activation by peanut decreased, and cytokine production
from peripheral blood mononuclear cells stimulated with peanut
was modulated and by 12 to 18 months, peanut specific IgE de-
creased and peanut specific IgG4 increased (Jones 2009). These
studies also demonstrated the high risk of adverse events associated
with OIT.
Randomised, double-blind, placebo-controlled studies of peanut
OIT are now ongoing. Important questions that need to be ad-
dressed before such an approach can be considered for routine clin-
ical use include issues to do with the effectiveness and cost-effec-
tiveness of OIT and the need to better appreciate the risks associ-
ated with treatment and how these can be minimised (Thyagarajan
2010).
Description of the intervention
As early as 1908, Schofield’s case report suggested that OIT can
lead to desensitisation in those with egg allergy (Schofield 1908).
Over the last hundred years, there has been considerable use of
immunotherapy, but the majority of this relates to the manage-
ment of people with allergic rhinitis who have pollen and other
inhalant allergies. It is typically delivered through the subcu-
taneous route and, more recently, by sublingual administration
(Calderon 2011a; Calderon 2011b; Radulovic 2010). Two decades
ago, small placebo-controlled trials of subcutaneous immunother-
apy in peanut allergy were undertaken. Although the results were
in some respects promising, the frequency of adverse events was
high (Oppenheimer 1992; Nelson 1997). OIT is now being in-
vestigated as an effective safer mode of immunotherapy in people
with peanut allergy.
Recent studies using oral (and sublingual) immunotherapy appear
to be promising. OIT is in particular of considerable interest as
a possible treatment for peanut allergy (Sheikh 2010). The aim
of treatment is initially to desensitise patients to peanut allergen,
which by increasing the threshold dose of exposure, reduces the
risk of reactions resulting from accidental ingestion whilst on treat-
ment (Burks 2008). The ultimate aim is to induce a state of cure
or tolerance, i.e. following the completion of OIT, allowing peo-
ple to eat peanut whenever they want and in whatever quantity
they want without developing symptoms. There is early evidence
that OIT can induce significant longer-term humoral and cellular
changes, which are suggestive of the development of immunolog-
ical tolerance (Clark 2009; Jones 2009).
How the intervention might work
OIT involves the administration of initially very small doses (usu-
ally micro or milligrams) of food allergen to food-allergic patients
in a controlled clinical setting. The dose of the administered food
allergen is then systematically increased until a maximum toler-
ated dose of food allergen is achieved (Jones 2009). Regular dos-
ing with this maximal dose is then maintained at home by the
patient. Successful desensitisation is thought to induce immuno-
logical tolerance by generating allergen specific IL-10 secreting
Tr1 and/or TGF-secreting Th3 regulatory T-cells (Sicherer 2006;
Moneret-Vautrin 2011). OIT is intended for patients with IgE-
mediated peanut allergy. Mechanisms of non-IgE mediated food
allergy are less well understood and currently OIT is not an ap-
propriate form of treatment for non-IgE mediated disease.
Why it is important to do this review
OIT is a treatment approach that has the potential to revolutionise
the management of people with peanut allergy. However, at present
there is still considerable uncertainty about the effectiveness and
safety of this approach (Sheikh 2010). There is therefore a need to
systematically identify, critically appraise and summarise available
evidence on the benefits and harms associated with OIT for the
management of people with peanut allergy.
O B J E C T I V E S
To determine the effectiveness and safety of OIT in people with
IgE-mediated peanut allergy. In this context, effectiveness means
that a patient who is sensitised to peanut and was previously un-
able to tolerate ingestion of peanut without developing symptoms
within minutes or hours, is, after undergoing peanut OIT, able
to ingest peanut and tolerate it without developing any clinical
symptoms.
M E T H O D S
Criteria for considering studies for this review
Types of studies
We were primarily interested in randomised controlled trials
(RCTs). However, anticipating a limited number of RCTs in this
area, we also planned to include studies using quasi-RCT and con-
trolled clinical trial (CCT) designs. Our working definition for
these designs are:
• RCTs: All participants are allocated at random (e.g. random
number generation, coin flips).
• Quasi-RCTs: The intervention is allocated in a way that is
not truly random: for example, allocation by birth, day of the
week, month of the year.
4Allergen-specific oral immunotherapy for peanut allergy (Review)
Copyright © 2012 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd.
• CCTs: Trials that contains at least two groups, one which
receives the treatment and one which acts as a comparison group.
The comparison group receives placebo/no treatment other than
usual care.
Types of participants
We were interested in studies conducted in children or adults
with confirmed peanut allergy. For the purposes of this review,
peanut allergy was defined as a history of systemic clinical symp-
toms within minutes to hours after the ingestion of peanut in
those with objective evidence of sensitisation to peanut protein
(Sicherer 2010b). Objective evidence of sensitisation consisted of
an elevated serum peanut-specific IgE (ImmunoCAP) level (using
cut-points defined by the centre/study) or a positive skin prick test
(SPT) response to peanut, where this was defined as a wheal of ≥3
mm than that produced by the saline control.
Types of interventions
We were interested in studies investigating peanut OIT compared
either with a placebo group, an alternative way of administering
desensitisation therapy or treatment with a peanut avoidance con-
trol group. We did not include studies which investigated OIT in
combination with another treatment if the effect of OIT could
not be evaluated independently from the additional treatment.
Types of outcome measures
We consider outcomes measured at any time period during or
after treatment. We only included studies which assessed either
one or both of our primary outcomes of interest, but we did not
restrict studies to those with pre-/post-intervention double-blind,
placebo-controlled oral food challenge (OFC) (Plaut 2009).
Primary outcomes
Our primary outcome measures of interest were:
• Increase in the amount of peanut that can be ingested and
tolerated while receiving OIT (i.e. evidence of desensitisation)
• Complete recovery from peanut allergy after completion of
OIT whether or not peanut is eaten (i.e. induction of
immunologic tolerance).
Secondary outcomes
Our secondary outcome measures of interest were:
• Changes in generic and disease specific quality of life
• Satisfaction (patients and carers)
• Frequency and severity of further reactions
• Adverse events
• Drop-outs
• Impact on co-morbidities
• Medication use
• Health care utilisation
• Cost-effectiveness
• Immunological changes suggestive of the induction of
tolerance (e.g. decreased peanut-specific IgE, increased peanut
specific IgG4, and modulation of cytokine production from
peanut-stimulated peripheral blood mononuclear cells).
Search methods for identification of studies
We conducted systematic searches for RCTs, quasi-RCTs and
CCTs regardless of language, geographical area or publication sta-
tus. The date of the last search of all databases was 21 January
2011.
Electronic searches
We searched the following databases: AMED, BIOSIS, CAB,
CINAHL, The Cochrane Library, EMBASE, Global Health,
Google Scholar, IndMed, ISI Web of Science, LILACS, MED-
LINE, PakMediNet, and TRIP. We restricted our searches to the
period 1990 to 2012, as peanut OIT was not studied prior to
1990.
MEDLINE search strategies are detailed in Appendix 1 and the
search strategies used for other databases are found in Appendix
2. We combined subject search strategies with adaptations of the
highly sensitive search strategy designed by the Cochrane Col-
laboration for identifying randomised controlled trials and con-
trolled clinical trials (as described in the Cochrane Handbook Ver-
sion 5.1.0., Box 6.4.b, Higgins 2011).
Searching other resources
In order to identify unpublished and ongoing work we searched
key relevant internet-based databases: Current Controlled Tri-
als (http://www.controlled-trials.com); ClinicalTrials.gov (http://
www.clinicaltrials.gov); and Australian New Zealand Clinical Tri-
als Registry (http://www.anzctr.org.au). We contacted experts in
the field for information on ongoing and unpublished work (Table
1). We also searched the references of all studies identified by the
above methods.
Data collection and analysis
Selection of studies
Two reviewers (UN and IV) independently reviewed the titles and
abstracts retrieved during the search process and selected all stud-
ies that potentially satisfied our inclusion criteria. Both review-
ers (UN and IV) then independently assessed the full text copies
5Allergen-specific oral immunotherapy for peanut allergy (Review)
Copyright © 2012 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd.
of these potentially eligible studies against the inclusion criteria.
Disagreements were resolved through discussion between UN and
IV and AS arbitrated in instances where an agreement was not
reached. For a PRISMA diagram of study selection, see Appendix
3.
Data extraction and management
We collected included study details using a form designed for this
purpose. Two reviewers (UN and IV) only included data if there
was an independently reached consensus. If no agreement was
reached, a third reviewer (AS) arbitrated.
The following data were, where available, independently extracted
from the included study:
• Trial methods
• Country and setting
• Participants (N, mean age, age range)
• Description of intervention
• Outcome measures (primary and secondary)
• Study withdrawal
• Adverse events.
Assessment of risk of bias in included studies
Risk of bias was assessed and documented following the domain-
based evaluation described in the Cochrane Handbook for SystematicReviews of Interventions 5.1.0 (Higgins 2011). We compared the
evaluations and discussed and resolved any inconsistencies and
disagreements. We assessed the following domains as low, high, or
unclear risk of bias:
• Random sequence generation
• Allocation concealment
• Blinding of participants and personnel
• Blinding of outcome assessment
• Incomplete outcome data
• Selective reporting
• Other bias.
Data synthesis
Due to finding only one included study, we were unable to perform
meta-analysis in this review. Had sufficient data been available, we
planned to use Review Manager for data analysis and quantitative
data synthesis. For dichotomous data, we planned to calculate
individual and pooled statistics as relative risks (RR) with 95%
confidence intervals (CI). For continuous data, we planned to
calculate individual and pooled statistics as mean differences (MD)
and/or standardised mean differences (SMD) with 95% CI. We
planned to conduct meta-analysis using the fixed-effect model.
Quantitative analyses of outcomes are, wherever possible, on an
intention to treat basis. If we had found sufficient studies, we
would have assessed evidence of publication bias graphically using
Funnel plots and statistically using Begg and Egger tests (Begg
1994; Egger 1997).
Subgroup analysis and investigation of heterogeneity
We planned to considerthe appropriateness of meta-analysis in the
presence of significant clinical or statistical heterogeneity. In future
iterations of this review, relevant heterogeneity will be tested for
using the I2 statistic and significant heterogeneity assumed if I2 is
greater than 40% (i.e. more than 40% of the variability in outcome
between trials could not be explained by sampling variation).
In future updates of this review, if data allow, we plan to undertake
subgroup analyses in relation to:
• Isolated peanut allergy vs. multiple food allergies
• Oral immunotherapy regimen (rush vs. standard)
• Duration of treatment
• Time since completion of treatment.
Sensitivity analysis
We did not find sufficient studies to conduct sensitivity analyses.
We planned to undertake sensitivity analyses for the allocation of
missing data by best and worst case analysis, and also to undertake
a sensitivity analysis on the basis of only including studies judged
to be at low risk of bias. We also planned to undertake sensitivity
analysis using random effects modelling, comparing the pooled
estimates with those derived from undertaking fixed-effect meta-
analysis.
Systematic review protocol
The methods used in this review were specified in advance and
documented in a protocol which has been published (Nurmatov
2011).
R E S U L T S
Description of studies
See: Characteristics of included studies; Characteristics of excluded
studies; Characteristics of ongoing studies.
Results of the search
Our searches identified a total of 746 titles, 16 of which we selected
for more detailed interrogation. See Appendix 3 for a study flow
diagram.
6Allergen-specific oral immunotherapy for peanut allergy (Review)
Copyright © 2012 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd.
Included studies
We found one small, US-based RCT (Varshney 2011) that en-
rolled 28 children aged 1 to 16 years: 19 in the intervention arm
and nine in the placebo arm. Subjects were patients with a clini-
cal history of peanut allergy and evidence of allergic sensitisation
to peanut protein. The active treatment was peanut flour, com-
pared with a placebo of oat flour. Patients with a history of severe
peanut anaphylaxis, moderate-to-severe persistent asthma, poorly
controlled atopic dermatitis, oat allergy (due to use as placebo)
or inability to discontinue antihistamines for a short period of
time were excluded from the study population. The average length
of oral immunotherapy (OIT) was 48 weeks: the initial day es-
calation phase lasted two days; the dose escalation period (with
home dosing and build-up visits) lasted approximately 44 weeks
and the maintenance phase lasted approximately four weeks. A
double-blind, placebo controlled oral food challenge (OFC) was
conducted at week 48. Adverse events were recorded and peanut-
specific IgE, IgG and IgG4 were measured. Secreted cytokine as-
says and T-cell analyses were conducted in subsets of patients.
See Characteristics of included studies for further details.
Excluded studies
We excluded 15 of the 16 studies we identified from screening,
as they did not meet our specified inclusion criteria. Eight stud-
ies were either case series or open trials without a control group
(Anagnostou 2011; Blumchen 2010; Buchanan 2006; Clark 2009;
Jones 2009; Moneret-Vautrin 2010; Nash 2008; Wassermann
2010), two were case reports (Aruanno 2009; Mansfield 2006),
two studied forms of immunotherapy other than OIT (Bird 2010;
Kim 2011), and three did not report either of our primary out-
comes (Hofmann 2009 and Weldon 2011 reported only on safety,
and Thyagarajan 2008 reported only immunological outcomes).
See Characteristics of excluded studies for further information.
Ongoing studies
Searches of clinical trials registries revealed eight studies of peanut
OIT for which results had yet to be published. Five of these
eight studies were RCTs: NCT00815035 and NCT01324401
compare OIT with placebo and OIT with control, respec-
tively; NCT01084174 compares OIT with sublingual im-
munotherapy; NCT00932282 compares two lengths of treat-
ment with a combination of anti-IgE therapy and OIT; and
ACTRN12608000594325 studies the combination of a probi-
otic and OIT, with the intervention group receiving OIT and a
probiotic and the control group receiving the same probiotic and
placebo OIT. Further details can be found in Characteristics of
ongoing studies.
Risk of bias in included studies
The one included RCT (Varshney 2011) was judged to be at low
risk of bias in all domains. Further details of the risk of bias as-
sessment can be found in the Characteristics of included studies
table.
Effects of interventions
Effectiveness of OIT on increasing peanut threshold
dose
During the initial day of treatment escalation, 26 out of 28 (93%)
individuals reached the maximum cumulative dose of 12mg of
peanut protein (equivalent to approximately 1/25th of a peanut)
or placebo. Two patients in the intervention arm did not reach
the 1.5 mg dose and were therefore deemed initial day escalation
failures.
Because of adverse events, three patients withdrew from the inter-
vention arm (i.e. the two escalation day failures and one additional
patient); only 16 participants therefore received the full course of
peanut OIT. All nine patients in the placebo arm completed the
trial. In those who completed the study, there was a significant
increase in the threshold dose of peanut allergen required to trig-
ger a reaction in those in the intervention arm compared to those
in the placebo arm at the 48 week OFC. All 16 participants who
received the full course of active treatment were able to ingest the
maximum cumulative dose of 5000 mg (reported by the authors
as equivalent to approximately 20 peanuts), compared to a me-
dian cumulative dose of 280 mg in the placebo group (range: 0 to
1900; P < 0.001), which equates to approximately one peanut.
Adverse events
Forty-seven per cent (n = 9) of subjects in the intervention arm
experienced clinically relevant adverse events during initial day
escalation. No placebo subjects had clinically relevant symptoms
during initial day escalation. During the build-up phase, peanut
OIT subjects had clinically relevant symptoms after 1.2% of 407
build-up doses. One subject treated with peanut OIT experienced
mild gastrointestinal symptoms during up-dosing. During home
dosing, one placebo group subject experienced adverse events, but
no further details about the nature of these symptoms were given.
At OFC, one peanut OIT-treated subject experienced mild up-
per respiratory symptoms and moderate urticaria. In the placebo
treated group, eight subjects experienced clinically relevant symp-
toms: one had gastrointestinal symptoms and oral pruritus and a
further three subjects had more severe symptoms, but no further
details about the nature of these reactions of these were available.
7Allergen-specific oral immunotherapy for peanut allergy (Review)
Copyright © 2012 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd.
Medication use
Nine (47%) of the OIT subjects received antihistamine treatment
during initial day escalation and two of these subjects required
epinephrine. None of the placebo subjects required treatment dur-
ing initial day escalation. During build-up dosing, none of the sub-
jects required medication. No detailed information about other
medication use during this phase was given. During home dosing,
one of the children in the placebo group required treatment with
epinephrine. During OFC, one of the peanut OIT treated sub-
jects required antihistamines. Three of the placebo group subjects
required treatment with epinephrine during OFC. No further de-
tails were given about the reason for the use of epinephrine.
Titrated skin prick test (SPT)
In the peanut OIT group, the titrated SPT size decreased from a
median of 7 mm (range: 5.5 to 15 mm) at baseline to 1.75 mm
(range: 0 to10 mm) by the time of the OFC, which was performed
after four weeks of maintenance therapy. In the placebo group,
there was a smaller reduction in the titrated SPT size from 7 mm
(range; 5.5 to 13 mm) at baseline to 4 mm (range: 0 to 12.5 mm)
at OFC.
Changes in peanut-specific IgE
Peanut-specific IgE was measured at the beginning of the study
and then at two, six and nine months and at OFC. The median
baseline peanut IgE levels in the peanut OIT and placebo groups
were 104 kU/L (range; 31 to 685 kU/L) and 57 kU/L (range;
20 to 188 kU/L), respectively (P = 0.02). After two months, the
median peanut-specific IgE of peanut OIT subjects increased to
308 kU/L (P < 0.01). At OFC, median peanut-specific IgE was
not significantly different from baseline. Placebo subjects showed
no changes.
Changes in peanut-specific IgG4
Peanut-specific IgG4 significantly increased from baseline at all
time-points in peanut OIT treated subjects, but did not change
in placebo subjects (P < 0.001).
Other immunological changes
Peanut OIT treated subjects had an initial increase in specific-IgE
(P < 0.01), but did not show any significant changes from baseline
by the time of the OFC.
Cytokines and T-cell parameters were measured at baseline, nine
months, and at the time of OFC in eight peanut subjects and nine
placebo subjects who had cultured peripheral blood mononuclear
cells (PBMCs) at these time points. At nine months and OFC, IL-
5 and IL-13 were significantly decreased from baseline in peanut
OIT subjects (P < 0.03). There was a transient increase in TGF-
β levels in peanut OIT subjects at 9 months (P = 0.03), but levels
returned to baseline by the time of the OFC. In placebo arm
subjects, there was no change in IL-5, IL-13 or TGF-β. There was
no significant change found in IL-10 or IFN-γ in either peanut
OIT or placebo subjects.
Drop-outs
Two subjects in the peanut OIT treated arm did not reach the
1.5 mg dose and therefore did not continue the study. During
the build-up phase, one additional peanut OIT subject withdrew
from the study after the first dose escalation because of mild gas-
trointestinal symptoms precluding further up-dosing.
Other outcomes
No other outcomes specified in the protocol were reported in the
included study.
D I S C U S S I O N
Summary of main results
This review identified one small RCT at low risk of bias. This
RCT demonstrated that it is possible to induce desensitisation in
children treated with allergen-specific peanut OIT, enabling a sub-
stantial increase in the amount of peanut to be safely consumed
whilst receiving OIT. There was a significant increase in threshold
dose of peanut allergen tolerated by children after peanut OIT; in
the intervention group, the children ingested the maximum cumu-
lative dose of 5000 mg (equivalent to approximately 20 peanuts),
whereas in the placebo group the mean amount of peanut allergen
ingested was 280 mg (range, 0 to 1900 mg, P < 0.001). Treat-
ment was associated with relevant immune-modulatory changes
suggesting that OIT may have longer-term benefits, but data were
not available on induction of tolerance. However, adverse events
associated with treatment were common and in some cases these
reactions were severe, raising important concerns about the safety
of the OIT regimen employed in this trial. There is no evidence
on the cost-effectiveness of this treatment approach. Concerns
around safety and cost-effectiveness mean that although OIT for
peanut allergy should at present be regarded as a promising line of
enquiry, it is not as yet appropriate for incorporating into clinical
care.
Overall completeness and applicability ofevidence
8Allergen-specific oral immunotherapy for peanut allergy (Review)
Copyright © 2012 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd.
Although the evidence identified in this review is consistent with
that reported in our earlier systematic review of peanut OIT case
series (Sheikh 2012), the only rigorous evidence on this subject
comes from one small eligible RCT in children (Varshney 2011).
There is therefore at present only limited evidence to inform care
provision and, given the concerns about safety, the applicability of
this evidence is limited. We found no evidence in relation to the
management of adults.
Quality of the evidence
The one included trial was well-conducted, but it was small and
involved children from only two centres (Varshney 2011), limiting
its external validity.
Potential biases in the review process
We have undertaken comprehensive searches of a range of data
sources. That said, there remains the possibility that we may have
failed to uncover some potentially eligible studies. We are not
aware of any other sources of bias in the review process.
Agreements and disagreements with otherstudies or reviews
The findings from our systematic review are broadly consistent
with other reviews on this subject (Fisher 2011; Sheikh 2012),
which suggest that whilst desensitisation can be achieved through
peanut OIT there is at present no evidence in relation to the
induction of tolerance. In a Phase I trial from the USA (Weldon
2011), 24 subjects aged 5 to 45 years old received 3777 doses of
OIT. Twenty-one out of 24 patients reported reactions; 85 per cent
of those were mild and resolved with antihistamines (abdominal
pain 28%, oropharyngeal pruritus 27% and lip pruritis 11%) and
three reactions were severe and resolved with epinephrine.
A U T H O R S ’ C O N C L U S I O N S
Implications for practice
Peanut OIT represents a promising, potentially disease-modifying
therapeutic approach for the management of IgE-mediated peanut
allergy. However, currently there is insufficient evidence in terms
of long-term effectiveness, safety and cost-effectiveness of peanut
OIT to recommend its routine use in clinical practice.
Implications for research
Further research is needed to better establish the effectiveness, sa-
fety, and cost-effectiveness of allergen-specific peanut OIT. In
particular, future trials need to establish whether clinical and im-
munologic tolerance can successfully be achieved after OIT is dis-
continued, and to understand the impact of treatment on the qual-
ity of life of patients and relevant family members. There is also
a need to ensure that the full age spectrum of patients affected by
peanut allergy is reflected in future trials. The results of on-going
trials will, in the future, shed light on long-term oral immune tol-
erance issues in subjects with IgE-mediated peanut allergy.
A C K N O W L E D G E M E N T S
We wish to thank to the Chief Scientist Office of the Scottish Gov-
ernment for their funding our previous grant “Establishing the ef-
fectiveness, cost-effectiveness and safety of oral desensitisation for
food allergy: a systematic review and meta-analysis of intervention
studies” (CZG/2/493).
We also thank Managing Editors from the Cochrane Tobacco
Addiction Group, Dr. Monaz Mehta, Lindsay Stead and Jamie
Hartmann-Boyce for their assistance during the review process.
R E F E R E N C E S
References to studies included in this review
Varshney 2011 {published data only}
Varshney P, Jones S, Scurlock AM, Perry TT, Kemper A,
Steele P, et al.A randomized controlled study of peanut oral
immunotherapy: clinical desensitization and modulation
of the allergic response. Journal of Allergy and Clinical
Immunology 2011;127:654–60.
References to studies excluded from this review
Anagnostou 2011 {published data only}
Anagnostou K, Clark A, King Y, Islam S, Deighton J,
Ewan P. Efficacy and safety of high-dose peanut oral
immunotherapy with factors predicting outcome. Clinical
& Experimental Allergy 2011;41(9):1273–81.
Aruanno 2009 {published data only}
Aruanno A, Nucera E, et al.Oral specific desensitisation
in food allergy and loss of tolerance after interruption of
the maintenance phase: five case reports. Allergy 2009;64
(Suppl.90):167.
Bird 2010 {published data only}
Bird JA, Pons L, Kulis M, Kemper AR, Laubach S, Kim
9Allergen-specific oral immunotherapy for peanut allergy (Review)
Copyright © 2012 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd.
E, et al.Double-blinded placebo controlled sublingual
immunotherapy (SLIT) trial for peanut allergy. Journal ofAllergy and Clinical Immunology 2010;125(2):AB20–78.
Blumchen 2010 {published data only}
Blumchen K, Ulbricht H, Staden U, Dobberstein K,
Beschomer J, Lopes de Oliveira LC, et al.Oral peanut
immunotherapy in children with peanut anaphylaxis.
Journal of Allergy and Clinical Immunology 2010;126:83–91.
Buchanan 2006 {published data only}
Buchanan A, Scurlock AM, Jones SM, Christie L, Althage
KM, Pons L, et al.Oral desensitization and induction of
tolerance in peanut-allergic children. Journal of Allergy andClinical Immunology 2006;117:S327.
Clark 2009 {published data only}
Clark AT, Islam S, King Y, Deighton J, Anagnostou K,
Ewan PW. Successful oral tolerance induction in severe
peanut allergy. Allergy 2009;64(8):1218–20.
Hofmann 2009 {published data only}
Hofmann AM, Scurlock AM, Jones SM, Palmer KP,
Lokhnygina Y, Steele PH, et al.Safety of a peanut oral
immunotherapy protocol in children with peanut allergy.
Journal of Allergy and Clinical Immunology 2009;124(2):
286–91.
Jones 2009 {published data only}
Jones SM, Pons L, Roberts JL, Scurlock AM, Perry TT,
Kulis M, et al.Clinical efficacy and immune regulation with
peanut oral immunotherapy. Journal of Allergy and Clinical
Imunology 2009;124(2):292–300.
Kim 2011 {published data only}
Kim EH, Bird JA, Kulis M, Laubach S, Pons L, Shreffler W,
et al.Sublingual immunotherapy for peanut allergy: clinical
and immunological evidence of desensitization. Journal ofAllergy and Clinical Immunology 2011;127(3):640–6.
Mansfield 2006 {published data only}
Mansfield L. Sussessful oral desensitisation for systemic
peanut allergy. Annals of Allergy, Asthma and Immunology2006;97(2):266–7.
Moneret-Vautrin 2010 {published data only}
Moneret-Vautrin DA, Petit N, Parisot L, Dumont P,
Morisset M, Beadouin E, et al.Efficiency and safety of oral
immunotherapy protocols in peanut allergy: Pilot study on
51 patients. Revue Française d’Allergologie et d’Immunologie
Clinique 2010;50(5):434–42.
Nash 2008 {published data only}
Nash SD, Steele PH, Kamilaris JS, Pons L, Kulis MD, Lee
LA, et al.Oral immunotherapy for children with peanut
allergy. Journal of Allergy and Clinical immunology 2008;
121:S136.
Thyagarajan 2008 {published data only}
Thyagarajan A, Jones SM, Pons L, Kulis M, Smith PB,
Steele PH, et al.Immunoglobulin changes using different
dosing regimens of peanut oral immunotherapy (OIT)
in peanut allergic patients. Journal of Allergy and Clinical
Immunology 2008;125(2):AB21.
Wassermann 2010 {published data only}
Wassermann RL, Mansfield LE, Gallucci AR, Hutteman
HR, Ruvalcaba AM, Long NA, et al.Office based oral
desensitization of patients with anaphylactic sensitivity to
foods is safe and effective. Journal of Allergy and Clinical
Immunology 2010;125:AB59(233).
Weldon 2011 {published data only}
Weldon B, Yu G, Neale-May S, Hunter TT, Nadeau K.
THe safety of peanut oral immunotherapy in peanut allergic
subjects in a single center trial. Journal of Allergy and
Clinical Immunology 2011;127(2):AB25.
References to ongoing studies
ACTRN12608000594325 {unpublished data only}
Study of the effectiveness of Probiotics and Peanut Oral
Immunotherapy (P-POIT) in inducing desensitisation or
tolerance in children with peanut allergy. Ongoing study
December 2008.
NCT00598039 {unpublished data only}
Oral Peanut Immunotherapy for Peanut Allergic Patients.
Ongoing study March 2003.
NCT00815035 {unpublished data only}
Oral Immunotherapy for Peanut Allergy. Ongoing study
June 2009.
NCT00932282 {unpublished data only}
Peanut Oral Immunotherapy and Anti-IgE for Peanut
Allergy. Ongoing study July 2009.
NCT01084174 {unpublished data only}
A Randomized, Double-Blind, Placebo-Controlled Pilot
Study of Sublingual/Oral Immunotherapy for the Treatment
of Peanut Allergy. Ongoing study March 2010.
NCT01259804 {unpublished data only}
Efficacy and Safety of High-dose Peanut Oral
Immunotherapy With Factors Predicting Outcome.
Ongoing study Jan 2008.
NCT01274429 {unpublished data only}
Peanut Oral Immunotherapy (OIT) - Initial Pilot Study in
Adults. Ongoing study Dec 2010.
NCT01324401 {unpublished data only}
Oral Peanut Immunotherapy. Ongoing study March 2011.
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C H A R A C T E R I S T I C S O F S T U D I E S
Characteristics of included studies [ordered by study ID]
Varshney 2011
Methods Randomised double-blind, placebo-controlled trial
Setting: University health centres, USA
Participants 28 children aged 1 to 16 years from local allergy and immunology clinics or surrounding
community physician offices (19 intervention, 9 control). Av. age 7 years
Inclusion criteria: clinical history of reaction to peanut within 60min of ingestion; peanut
CAP-FEIA >15 kU/L or >7 kU/L if a significant reaction occurred within 6m of enrol-
ment; positive skin prick test
Excluded if: history of severe peanut anaphylaxis; moderate to severe persistent asthma;
poorly controlled atopic dermatitis; oat allergy; or inability to discontinue antihistamines
during OFC or skin testing
Interventions Oral immunotherapy for approx. 48wks (home dosing/build-up visits approx. 44wks;
maintenance phase approx. 4wks; OFC at wk 48)
Active = peanut flour; placebo = oat flour
Initial day escalation phase: Day 1: dosing started at 0.1 mg peanut protein or placebo,
approx. doubled every 30min until 6 mg reached/subject had symptoms. Day 2: Dosing
began at highest tolerated dose during day 1
Home dosing: Children instructed to ingest each dose mixed in a food vehicle daily
Dose escalations: Every 2 wks, doses increased 50 - 100% until 75 mg dose, 25-33% until
400 mg maintenance dose reached
Maintenance phase: Ingest 400 mg dose daily for 1m
Outcomes Double-blind, placebo controlled OFC (increasing doses of peanut or oat flour every 10
to 20mins up to cumulative dose of 5000 mg); adverse events; peanut-specific IgE, IgG,
and IgG4; secreted cytokine assays and T-cell analyses in subset of participants
Notes Small sample size
Participants followed up after wk 48, investigating long-term immune tolerance (data
not yet available)
Risk of bias
Bias Authors’ judgement Support for judgement
Random sequence generation (selection
bias)
Low risk “A randomization table was generated to
assign subjects in a 2:1 ratio to receive
peanut flour or placebo”
Allocation concealment (selection bias) Low risk “Allocation was performed before enrol-
ment and saved in a locked database acces-
sible only by laboratory personnel to keep
clinical staff and subjects unaware of up-
13Allergen-specific oral immunotherapy for peanut allergy (Review)
Copyright © 2012 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd.
Varshney 2011 (Continued)
coming assignments”
Blinding of participants and personnel
(performance bias)
All outcomes
Low risk “Investigators, subjects, and families re-
mained blind to the assigned intervention
as well laboratory studies until completion
of the food challenge”
Blinding of outcome assessment (detection
bias)
All outcomes
Low risk “Challenges were administered by a nurse
or physician who was also blind to testing
materials”
Incomplete outcome data (attrition bias)
All outcomes
Low risk Final data excludes 3 participants from
treatment group who were removed from
the study before completion due to adverse
events
Selective reporting (reporting bias) Low risk All prespecified outcomes reported
m: month; min: minute; OFC: oral food challenge; OIT: oral immunotherapy; wk(s): week(s)
Characteristics of excluded studies [ordered by study ID]
Study Reason for exclusion
Anagnostou 2011 Open trial without a control group
Aruanno 2009 Case reports
Bird 2010 Sublingual immunotherapy (not a study of OIT)
Blumchen 2010 Case series
Buchanan 2006 Case series
Clark 2009 Open trial without a control group
Hofmann 2009 Immunological outcomes only (tolerance and desensitisation not measured)
Jones 2009 Case series
Kim 2011 Sublingual immunothertapy (not a study of OIT)
Mansfield 2006 Case report
14Allergen-specific oral immunotherapy for peanut allergy (Review)
Copyright © 2012 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd.
(Continued)
Moneret-Vautrin 2010 Open trial without a control group
Nash 2008 Case series
Thyagarajan 2008 Immunological outcomes only (tolerance and desensitisation not measured)
Wassermann 2010 Case series
Weldon 2011 Safety analysis only (tolerance and desensitisation not measured)
Characteristics of ongoing studies [ordered by study ID]
ACTRN12608000594325
Trial name or title Study of the effectiveness of Probiotics and Peanut Oral Immunotherapy (P-POIT) in inducing desensitisation
or tolerance in children with peanut allergy
Methods Randomized controlled trial
Participants Approx. 90 children aged 1 to 10 with confirmed diagnosis of peanut allergy and no history of severe
anaphylaxis to peanut
Interventions Intervention: Lactobacillus rhamnosus GG (LGG) + peanut OIT
Control: LGG + placebo OIT
LGG: 2x10E10 daily for 18m
OIT: 18m. Modified rush phase: increasing doses, starting at 0.1 mg peanut protein, doubling every 30min
to reach dose of 12 mg of peanut protein. Build-up phase: daily dose starting at 24 mg, increased every 2wks
until dose of 2 g is reached (expected to take 8m). Dose then maintained at 2 g for approx 10m. Same for
placebo OIT
Outcomes Primary: increase in tolerance by combining peanut OIT with probiotics
Secondary: Desensitisation, immunologic changes
Starting date December 2008
Contact information A/Prof Mimi Tang: [email protected]
Notes Expected completion date not specified
15Allergen-specific oral immunotherapy for peanut allergy (Review)
Copyright © 2012 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd.
NCT00598039
Trial name or title Oral Peanut Immunotherapy for Peanut Allergic Patients
Methods Safety/efficacy study, open label, no control group
Participants Approx. 40 subjects 1 to 16 years old with diagnosed peanut allergy. Subjects with history of severe anaphylaxis
to peanut excluded
Interventions Peanut OIT (open intervention), no further details provided
Outcomes Primary: Double-blind, placebo controlled OFC and second food challenge 1m after being off of peanut
Secondary: IgE to peanut decrease below a level of 2
Starting date March 2003
Contact information Contact information not provided. PI: Wesley Burks, MD, Duke University
Notes Est. study completion date July 2012
NCT00815035
Trial name or title Oral Immunotherapy for Peanut Allergy
Methods Randomized, double-blind, crossover safety/efficacy study
Participants Approx. 60 participants aged 1 to 6 years with presence of peanut-specific IgE and history of significant
clinical symptoms occurring within 60mins of ingesting peanuts. Participants excluded if they have history
of severe anaphylaxis to peanut
Interventions Intervention: Peanut OIT
Control 1: Placebo OIT
Control 2: No treatment
Peanut OIT and placebo: modified rush immunotherapy on day 1 followed by daily dose with increase in
dose at least every 2 wks up to maintenance dose of 4 g
Outcomes Primary: lower risk of anaphylactic reactions, long-term tolerance
Secondary: molecular level effect on humoral activity/response
Starting date June 2009
Contact information Contact information not provided. PI: Wesley Burks, MD, Duke University
Notes Est. completion date Nov 2013
16Allergen-specific oral immunotherapy for peanut allergy (Review)
Copyright © 2012 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd.
NCT00932282
Trial name or title Peanut Oral Immunotherapy and Anti-IgE for Peanut Allergy
Methods Randomized, open label safety/efficacy study
Participants Approx. 10 subjects aged 12 or older with peanut-specific IgE and history of clinical symptoms occurring
within 60mins of peanut ingestion. Participants with history of severe anaphylaxis to peanut not included
Interventions Peanut OIT + omalizumab (anti-IgE): 12m vs 24m maintenance therapy
4m omalizumab treatment before peanut OIT, omalizumab continued until 1m post maintenance therapy.
Initial desensitization phase over 2d to goal of 950 mg peanut powder followed by build up phase over 4m
to goal maintenance dose of 8000 mg peanut powder
Outcomes Primary: Tolerate a dose of 20gm peanut flour during OFC
Secondary: Incidence of adverse events during initial escalation and build up phase
Starting date July 2009
Contact information [email protected]; [email protected]
Notes Est. completion date July 2014
NCT01084174
Trial name or title A Randomized, Double-Blind, Placebo-Controlled Pilot Study of Sublingual/Oral Immunotherapy for the
Treatment of Peanut Allergy
Methods Randomized, double-blind, parallel assignment safety/efficacy study
Participants 30 subjects aged 6 to 21 with clinical history of peanut allergy, confirmed by peanut specific IgE and skin
prick test
Interventions Active sublingual immunotherapy (SLIT) for peanut allergy + placebo peanut OIT vs active peanut OIT +
placebo SLIT
SLIT and OIT dose increases for 16wks, then daily maintenance dose for 12m
Outcomes Primary: 10 fold increase in tolerance
Secondary: Adverse events, changes in clinical and mechanistic endpoints, peanut tolerance
Starting date March 2010
Contact information Contact information not provided, PI: Robert Wood, MD, Johns Hopkins University
Notes Est. completion date Jan 2012
17Allergen-specific oral immunotherapy for peanut allergy (Review)
Copyright © 2012 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd.
NCT01259804
Trial name or title Efficacy and Safety of High-dose Peanut Oral Immunotherapy With Factors Predicting Outcome
Methods Open pilot study, no control group
Participants 22 children with peanut allergy aged 7-17
Interventions Peanut OIT: gradual updosing with every 2 wks (8-38wks) to 800 mg protein (5 peanuts/day); then 30wks
maintenance
Outcomes Primary: Pass/fail peanut challenge
No secondary outcomes listed
Starting date Jan 2008
Contact information Contact information not provided. PI: Andrew T. Clark, Cambridge Biomedical Campus, University of
Cambridge
Notes Est. completion date Jan 2012
NCT01274429
Trial name or title Peanut Oral Immunotherapy (OIT) - Initial Pilot Study in Adults
Methods Open label, no control group
Participants Approx. 20 adults aged 18 to 50 with diagnosis of peanut allergy or clinical history of peanut allergy, positive
SPT, and peanut-specific IgE. Subjects with severe anaphylaxis to peanut excluded
Interventions Peanut OIT (no control).
Begins with modified rush (0.1 mg of peanut protein, dose doubled every 30min up to 6 mg peanut protein)
. Dose given daily, escalation visits every 2wks. Maintenance phase starts at 2300 mg, taken daily
Outcomes Primary: lower risk of anaphylactic reactions in adults, long-term tolerance
Secondary: molecular level effect on humoral activity/response in adults
Starting date Dec 2010
Contact information [email protected]; [email protected]
Notes Est. completion date Sept 2014
18Allergen-specific oral immunotherapy for peanut allergy (Review)
Copyright © 2012 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd.
NCT01324401
Trial name or title Oral Peanut Immunotherapy
Methods Randomized, open-label, parallel-assignment safety/efficacy study of clinical symptoms after ingestion of
peanuts
Participants 32 subjects 7 to 12 years old with diagnosis of peanut allergy via skin prick test and history. Excludes
participants with history of severe anaphylactic reaction to peanut requiring treatment with 2+ administrations
of epinephrine or hospitalisation
Interventions Intervention: Peanut OIT, daily escalating dosages as determined in modified rush phase, escalated until daily
dose of 4000 mg is reached
Control: No treatment
Outcomes Primary: tolerance
Secondary: desensitisation; adverse events
Starting date March 2011
Contact information [email protected]; [email protected]
Notes Est. completion date August 2012
OIT: oral immunotherapy; m: month; min: minutes; wk(s): week(s)
19Allergen-specific oral immunotherapy for peanut allergy (Review)
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D A T A A N D A N A L Y S E S
This review has no analyses.
A D D I T I O N A L T A B L E S
Table 1. List of experts contacted
Name of author Country
Dr. Egidio Barbi Italy
Dr. Kirsten Beyer Germany
Prof. Wesley Burks USA
Dr. Andrew T Clark UK
Dr. Ernesto Enrique Spain
Dr. Mansouri Iran
Dr. Paolo Meglio Italy
Professor Moneret-Vautrin DA France
Dr. Martine Morisset France
Prof. Bodo Niggemann Germany
Prof. Giovanni Pajno Italy
Prof. G Patriarca Italy
Dr. Lydia Zapatero Remon Spain
Dr. Fernandez-Rivas Spain
Prof. Robert A Wood USA
20Allergen-specific oral immunotherapy for peanut allergy (Review)
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A P P E N D I C E S
Appendix 1. Medline search strategy
Nr. Search term
1 Peanut Hypersensitivity (MESH)
2 Peanut allerg*
3 Peanut-allergic
4 Arachis hypogaea allergy
5 Food allergy
6 Food hypersen*
7 1 OR 2 OR 3 OR 4 OR 5 OR 6
8 Desensitization, immunologic (MESH)
9 Desensiti*
10 Immunotherapy (MESH)
11 Immunotherapy
12 Oral immunotherapy
13 Oral desensiti*
14 Rush immunotherapy
15 Specific oral tolerance induction
16 8 OR 9 OR 10 OR 11 OR 12 OR 13 OR 14 OR 15
17 Analytical stud*
18 Intervention studies (MESH)
19 Experimental stud*
20 Etiology
21 Trial
22 Clinical trial (MESH)
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(Continued)
23 Clinical trial
24 Controlled clinical trial
25 Uncontrolled trial
26 Randomized controlled trial (MESH)
27 Randomi* controlled trial
28 Quasi-randomi* controlled trial
29 Non-randomi* trial
30 Placebos (MESH)
31 Random allocation (MESH)
32 Double-blind method (MESH)
33 Double-blind design
34 Single-blind method
35 Random*
36 Controlled before after design
37 Interrupted time series
38 Case series
39 17 OR 18 OR 19 OR 20 OR 21 OR 22 OR 23 OR 24 OR 25 OR 26 OR 27 OR 28 OR 29 OR 30 OR 31 OR 32 OR 33
OR 34 OR 35 OR 36 OR 37 OR 38
40 7 AND 16 AND 39
Appendix 2. Other databases search strategy
Search terms for (The Cochrane Library, LILACS, TRIP, CINAHL, ISI Web of Science, BIOSIS, PakMediNet, IndMed, Google
Scholar) 1990-2012
(peanut allergy or arachis hypogaea or legume* or nut allergy or food hypersensitivity or food allergy)
AND
(immunologic, desensiti* or immunotherapy or oral immunotherapy or oral desensiti* or specific oral tolerance induction or oral
tolerance induction)
AND
(intervention stud* or experimental stud* or trial or clinical trial* or controlled clinical trial or random* or randomi* controlled trial
or quasi randomi* or non randomi* or random allocation or single blind method or double blind method or triple blind method)
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Appendix 3. PRISMA flow diagram of study selection
Figure 1
Figure 1. PRISMA search flow diagram. Top level does not include searches that returned no results
(AMED, PakMediNet, IndMed, LILACS).
H I S T O R Y
Protocol first published: Issue 2, 2011
Review first published: Issue 9, 2012
C O N T R I B U T I O N S O F A U T H O R S
UN developed the protocol, undertook searches, study selection, critical appraisal and drafting the review. IV developed the protocol,
undertook searches, study selection, critical appraisal and commented on drafts of the review. GD helped with the design of the review
protocol and commented on drafts of the final review. FER helped with the drafting the review protocol and commented on drafts
of the review manuscript. AS conceived this review, oversaw all aspects of the development of the protocol and the conducting of the
review and commented on drafts of this review.
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D E C L A R A T I O N S O F I N T E R E S T
None known.
S O U R C E S O F S U P P O R T
Internal sources
• No sources of support supplied
External sources
• Chief Scientist’s Office of the Scottish Government, UK.
D I F F E R E N C E S B E T W E E N P R O T O C O L A N D R E V I E W
In the protocol, we implied but did not clearly specify that this review was focused on studying allergen-specific OIT; the title and
background have now been revised to better reflect the focus of this work. We have removed a reference from the protocol (Li 2001).
24Allergen-specific oral immunotherapy for peanut allergy (Review)
Copyright © 2012 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd.